Best Microbiology Laboratory Techniques in Pharmaceuticals

  • Published on: Dec 16, 2020

Microbiology laboratory techniques have developed steadily over many years, thanks to the dedication and lifetime work of many professionals.

Today, microbiology laboratory techniques have become a complex and sophisticated component of pharmaceutical quality control campaigns.

These techniques are essential to prove the safety, purity, potency, and sterility of medicinal products.

Microbiology laboratory techniques for Laminar flow cabinets

Laminar flow and biohazard cabinets are the most widely used working stations for Microbiologists who have to carry out most of their tricky work inside those cabinets for their safety and their laboratory environments.   

Do you know how Laminar flow cabinet Works?

Room air is taken in from the top of the cabinet through a disposable pre-filter with 85% arrestance (a measure of removing dust from the air).

This serves to trap larger particles and increase the life of the main filter. Air is forced evenly across the HEPA or ULPA filter(s).

The result is a stream of clean laminar air within the cabinet’s work zone, which dilutes and flushes all airborne contaminants from the interior.

The purified air travels in laminar lines across the cabinet’s working zone in a vertical, unidirectional stream and leaves the main work chamber across the entire open front of the cabinet.

A nominal filter face velocity of 0.45 m/s or 90 fpm ensures that the velocity is low enough to minimize air turbulence and possible entrapment from the room.

Laminar Flow Cabinet
Laminar Flow Cabinet
Laminar Flow Cabinet airflow
Laminar Flow Cabinet airflow

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What are the airflow patterns used in a laminar flow cabinet?

In a laminar flow cabinet, there could be two types of airflow:

Downward flow: The air filtration system is located in the cabinet’s ceiling, and low-velocity HEPA-filtered “laminar” air is blown downwards.

Cross (or horizontal) flow: The air filtration system is located in the cabinet’s rear wall. HEPA air is blown horizontally from the rear wall towards the open front of the cabinet.

Primary Vs. Secondary Air

Primary air: Air blown from the air filtration system before contacting any surface.

Secondary air: Air blown from the air filtration system that has contacted a surface, for example, equipment, material, or the operator’s hands.

Which air materials should be exposed to during aseptic manipulations in a Laminar flow?

Aseptic manipulations should only be conducted in primary air within a Laminar flow or Biological Safety Cabinet to reduce the risk of adventitious agent contamination (for example, bacteria, fungi, or contaminants from the environment, including the operator).

What are the airflow patterns of a biological safety cabinet?

There are different classes and types of biological safety cabinets.

For example, Class II cabinets have downward airflows and provide sample and operator protection.

In addition to a laminar flow cabinet, air in a biological safety cabinet is drawn into the cabinet via a grill at the front where the operator sits to contain air blown from the cabinet.

How are materials transferred to and from safety cabinets?

Transferring materials into a safety cabinet involves following steps

– Wrap materials to avoid contamination.

– Sterilize the external surface if wrapping is not possible.

– Unload materials directly into the cabinet.

– Seal materials and transfer them directly to a Biological Hazard bag, as they may be contaminated.

Transferring materials into a safety cabinet

When transferring materials into a Laminar flow or Biological Safety Cabinet, the risk is that the materials may be contaminated with adventitious agents. Therefore, materials should be sterilized before the transfer.

Materials for sterilization should be wrapped. This will enable the removal of an outer layer when the material is transferred to environments of higher quality or lower bioburden.

For example, when transferring items from a laboratory into a Laminar flow or Biological Safety Cabinet.

If wrapping materials is not possible, the external surface should be thoroughly sanitized, using physical contact (friction) with the surface in conjunction with a chemical sanitant lethal to the microorganisms on the material’s surface.

Transferring materials out of a safety cabinet

When transferring materials out of a Laminar flow or Biological Safety Cabinet, the risk is that the materials may be contaminated, thus contaminating the environment and operator (a safety concern).

Therefore, all materials removed from the cabinet should be sealed before removal and transferred directly to a Biological Hazard bag. It will then undergo controlled destruction by an operator wearing gloves, which should also be placed in another Biological Hazard bag.

Microbiology laboratory techniques in media preparation

Importance of media

Media is a nutrient used for growing bacteria, yeasts, and molds in containers. If the media is poorly prepared, it may not support growth, and important microorganisms may not be detected.

A test may read “negative,” but the result may be due to poor media preparation that has not supported growth.

Or, a test may read “positive” because the media itself was contaminated.

It is very important to test and prepare media strictly following the laboratory procedures carefully.

Microbiology media and reagents
Microbiology media and reagents

How do you store dehydrated Media?

General guidelines for the storage of dehydrated media include:

– Store media in tightly capped bottles in a cool, dry place protected from light. Monitor the storage temperature.

– Keep a limited supply on hand – turn over the stock regularly.

– Practice first-in, first-out (FIFO) – use older stocks first.

– Do not exceed the supplier’s expiration date.

– Be alert to any physical signs of deterioration, such as discoloration.

What key points should be noted during media preparation?

If you have read and understood the importance of media as a commonly used microbiology lab technique, please take enough precautions while preparing these media.

Use the steps below:

i. Select and document the correct media and check that it is within its expiry date.

ii. Prepare the media as per the manufacturer’s instructions.

iii. Check key measurements, such as pH.

iv. Prepare the media with water with an appropriately low bioburden.

v. Prepare the media shortly before sterilization to ensure a low bioburden challenge to the sterilization process.

What issues may arise during Heat Sterilization?

When sterilizing by heat, there is a potential concern that the nutrients or other ingredients that maintain the media’s growth-promoting properties may be destroyed if the media is overcooked.

If it is undercooked, it may not be sterilized properly.

The former would lead to a false negative and positive result.

Therefore, the manufacturer’s instructions about sterilizing conditions must be strictly followed.

Sterilization should be conducted using specific validated sterilizing cycles demonstrating that the media has maintained all of its required characteristics (fertility, growth, sterility, pH, selective inhibition).

What issues may arise during the filtration of Sterilizing Media?

Filtration is conducted using a bacterial retentive filter and should be performed following the manufacturer’s instructions.

Filtration may remove certain ingredients in the sterilizing process, so it is important to confirm the fertility and key characteristics of media post-filtration as a quality control test.

Importance of media and reagents in microbiology techniques

Microbiological media and test reagents should be tested for their performance. This should be done for pre-prepared media and media formulated in the laboratory.

Some tips for media preparation and quality control are listed below:

– Develop SOPs and worksheets to record the preparation

– Ensure there is documented traceability from source media to each batch

– Perform quality control on dehydrated media when purchased

– Media can deteriorate if not stored correctly. Protect from exposure to moisture, heat, or oxidation. Please don’t use past expiry periods.

– Accurately weight dried media on calibrated balances

– Accurately weigh or measure water in calibrated glassware

– Ensure the glassware is not contaminated or dirty

– Use purified clean water to reconstitute the media – do not use tap water

– Ensure complete mixing or solubilization of ingredients during preparation

– Pre-melt agar before sterilization to ensure even mixtures

– Validate the autoclave to ensure there is no overheating of media during sterilization – overheating may reduce media efficacy

– Accurate pH of media is important for optimum performance

– Perform quality control on finished prepared media

What quality control tests are done to ensure media performance?

Some key quality control tests are carried out on media by microbiology laboratories in pharmaceuticals.

A few of those are listed below:

Fertility:

The ability to support micro-organisms that the media is claimed to support.

Micro-organism selectivity:

The ability of media to selectively support specific organisms’ growth while inhibiting other microorganisms’ growth.

pH:

The pH of the media is important because some bacteria will only grow well in a narrow pH range.

Defined media characteristics:

When acid is produced, the resulting change in pH may cause the media to change color. This color change can aid in the identification of organisms.

Sterility:

The media must be sterile (free of all living organisms) before it is used. Conduct a sterility test to verify that it is suitable before using it.

How to determine the effectiveness of Media?

The effectiveness of media depends on its ability to support the growth of organisms. Like all active materials, dried media loses its effectiveness and stability over time.

It is important to:

– Store dried and prepared media under approved temperature conditions

– Never use expired media

– Always test incoming media supplies before use

The media’s effectiveness depends on its fertility (ability to support growth), which depends on its stability.

The key factors affecting stability are time and temperature. Therefore, the storage conditions in which the media is stored (temperature and sometimes humidity) and the duration for which it is stored (it must be within the expiry date) are critical to maintaining its characteristics, including fertility.

Importance of traceability

Media lot number traceability is critical because:

– It gives us the means to trace problems in media batches and identify other batches that may be affected.

– It provides the ability to trace information supporting batches of media manufactured.

– It is a GLP requirement.

210 SOPs, 197 GMP Manuals, 64 Templates, 30 Training modules, 167 Forms. Additional documents included each month. All written and updated by GMP experts. Checkout sample previews. Access to exclusive content for an affordable fee.

Reading exposure plates – another microbiology laboratory technique

Pharmaceutical companies must regularly monitor the environment for bacteria, yeasts, and molds, which would alert personnel to poor sanitation practices, air supply problems, or even possible product risks.

Several different means can be used to do this:

– Air sampling using a volumetric air sampler

– Exposure (settle) plates

– Surface swabs

– Contact plates or RODACs

This monitoring helps you understand the number and types of undesirable microorganisms in the environment and near your products. If the numbers exceed expectations, investigations should be carried out.

What is involved in environmental monitoring?

1. Exposure plates

Exposure Plates are sterile petri dishes containing sterile media. They are utilized to detect the number of microorganisms in the environment.

They rely on microorganisms in the air or attached to particles in the air, falling onto the media surface.

After exposure to the environment for a defined period (often 2 hours), the plates are closed and then incubated for a defined period. The number of microorganisms is then counted.

2. Air sampling method

– A common method.

– “Active” sampling of air volume.

– Easy to use – the machine must be calibrated.

– Microbes are drawn into the sampler by an Impeller.

– Sample 10-20 minutes for 1 cubic meter of air.

– Incubate strip at 20 -25°C and 30 – 35°C for 3 – 5 days.

– Use selective strips for bacteria and yeast/mold.

3. Settle Plate method

– Most common method

– Easy to use and cheap

– “Passive” sampling

– Particle must fall on plate

– Bacteria must be attached to a particle

– Easy to influence the result

– Open for 2 – 4 hours

– Way dry out

– Incubate 20 -25°C and 30 – 35°C for 3 – 5 days

– Use selective plates for bacteria and yeast/mold

What are microbiological plating techniques?

There are two basic techniques for enumerating (counting) colonies in a pharmaceutical quality control test:

1. Membrane filtration (MF) technique:

The membrane filtering technique is most useful when looking for low numbers of colonies in a sample, such as less than 10 cfu in 100 mL of highly purified water.

The technique allows you to filter large volumes and trap the organisms on the filler face.

The filter can then be placed onto a plate, allowing the organisms to grow into countable colonies.

2. Direct plate method:

The Direct Plate method is preferred when detecting relatively large numbers of organisms in a sample volume.

In this method, 0.2mL of the sample is directly spread or poured onto a sterile plate and incubated.

This method is more direct and easier to use; however, its sensitivity is not as good as the Membrane Filtration method.

What are the advantages of the membrane filtration technique?

The advantages of the membrane filtration technique include:

– Large sample volumes can be tested

– Low organism numbers can be detected (it is a sensitive method)

– Allows isolation and enumeration of discrete colonies of bacteria

– Allows for removal of bacteriostatic or bactericidal agents by washing of the filter

Membrane filtration test method

– Filter must be sterile hydrophobic 0.45um – 47 – 50mm

– 3 by 100mL final washes of filter – less if validated

Incubation conditions

5 days @ 30 – 35°C for Total Aerobic Microbial Count (TAMC).

Direct plate techniques

There are two basic direct plating techniques used:

1. Spread Plate technique: a sample is aseptically “spread” directly onto the agar surface.

2. Pour Plate technique: a sample is applied to the plate, then agar is poured thinly over the top.

The PP technique can enumerate organisms using three different media: Nutrient Agar (NA), R2A agar, and SAB agar.

All of these media are generally non-selective: they supply nutrients and support the growth of various heterotrophic microorganisms.

– Incubation Conditions – Pour Plate Method

– 5 days @ 30 – 35°C for TAMC

– 5 days ® 20 – 25°C for yeasts and molds

Reliability of exposure (settle) plates in environmental monitoring

The following factors affect the reliability of exposure plates:

1. The media type selected for different micro-organisms (SAB plates support mold and yeast, and nutrient agar supports most bacteria).

2. Plate exposure time (1, 2, or 4 hours) affects the number of organisms trapped.

3. Time and temperature of incubation either supports or retards colony growth.

How do you read plates, count colonies, and record results?

After the incubation, the plates are removed, and the colonies are counted.

The number of colonies is recorded, as should be the units. The media type determines the colony count units.

For example, 38 colony forming units (cfu) on Saboraud Dextrose Agar is 38 cfu fungi per x hours of plate exposure to the environment.

– Reading or counting plates after incubation requires a good technique,

– Read under 10-15X microscope.

– Use a grid pattern approach.

– Be careful not to count colonies twice.

– If the count is > 300, record it as “Too Numerous to Count” or TNTC.

– Check for confluent (spreading) growth across the plate.

– Record the result and the morphology of the major types.

210 SOPs, 197 GMP Manuals, 64 Templates, 30 Training modules, 167 Forms. Additional documents included each month. All written and updated by GMP experts. Checkout sample previews. Access to exclusive content for an affordable fee.

Picture of Author: Kazi Hasan

Author: Kazi Hasan

Kazi is a seasoned pharmaceutical industry professional with over 20 years of experience specializing in production operations, quality management, and process validation.

Kazi has worked with several global pharmaceutical companies to streamline production processes, ensure product quality, and validate operations complying with international regulatory standards and best practices.

Kazi holds several pharmaceutical industry certifications including post-graduate degrees in Engineering Management and Business Administration.

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